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Associate

Roberto Laos

Education

  • BS in Chemistry. Pontificia Universidad Catolica, Peru (2000)
  • License Diploma in Chemistry. Pontificia Universidad Catolica, Peru (2001)
  • MS in Polymer Science and Engineering. University of Massachusetts Amherst (2003)
  • PhD in Chemistry. University of Florida (2015)

Research summary

My research is focused on the in vitro evolution of DNA polymerases. My goal is to produce DNA polymerases able to efficiently incorporate non-standard nucleic acids (AEGIS), which are designed and synthesized by my colleagues at the FFAME.

The compartmentalized self-replication (CSR) method allows us to isolate different phenotypes of polymerases in microdroplets that contain, on average, one gene per microdroplet. This system resembles natural selection, as the genes and the molecules they encode are separated from each other in compartments (cells). Our in vitro selection experiments allow us to select among 2x108 different genes in a volume of less than 1 mL.

Awards

  • Coca Cola Eco-efficiency Award (First Place, 2003)

Recent Publications

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Alternative Watson-Crick Synthetic Genetic Systems
Steven A. Benner, Nilesh B. Karalkar, Shuichi Hoshika, Roberto Laos, Ryan W. Shaw, Mariko Matsuura, Diego Fajardo, and Patricia Moussatche
Cold Spring Harb Perspect Biol , Cold Spring Harbor Laboratory Press (2016) doi: 10.1101/cshperspect.a023770
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DNA polymerases engineered by directed evolution to incorporate non-standard nucleotides. Frontiers in Microbiology
Laos, R., Thomson, J. M., & Benner, S. A.
Frontiers in Microbiology (2014) 5, 565. http://doi.org/10.3389/fmicb.2014.00565
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Directed Evolution of Polymerases To Accept Nucleotides with Nonstandard Hydrogen Bond Patterns
Laos R, Shaw R, Leal NA, Gaucher E, Benner S.
Biochemistry (2013) 52, 5288-5294
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Engineered DNA Polymerases
K. Murakami and M.A. Trakselis (eds.)
Nucleic Acid Polymerases, Nucleic Acids and Molecular Biology , Springer-Verlag Berlin Heidelberg (2013)

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Solution H-1 NMR confirmation of folding in short o-phenylene ethynylene oligomers
Jones, TV; Slutsky, MM; Laos, R; de Greef, TFA; Tew, GN
J. Am. Chem. Soc. 127 (49) 17235-17240 (2005)
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