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Ryan Shaw


  • BS in Chemistry/Biochemistry. Ohio University (1998)
  • PhD in Biochemistry and Molecular Biology. University of Florida (2004)

Research summary

I am interested in developing new enzymes through the process of in vitro directed evolution. My work focuses on using water-in-oil emulsions to compartmentalize chemical reactions, specifically polymerase chain reactions that amplify target DNA sequences. By compartmentalizing the reaction we can link the activity of the polymerase (phenotype) to the template DNA that encodes that enzyme (genotype). Using this system we are searching for polymerase variants that can better utilize AEGIS components and for polymerases that can fulfill other needs in biotechnology, such as novel DNA sequencing technologies.

Recent Publications


Assays To Detect the Formation of Triphosphates of Unnatural Nucleotides: Application to Escherichia coli Nucleoside Diphosphate Kinase
Mariko F. Matsuura, Ryan W. Shaw, Jennifer D. Moses, Hyo-Joong Kim, Myong-Jung Kim, Myong-Sang Kim, Shuichi Hoshika, Nilesh Karalkar, and Steven A. Benner
ACS Synthetic Biology , American Chemical Society (2016) 5 (3), pp 234-240 DOI: 10.1021/acssynbio.5b00172


Alternative Watson-Crick Synthetic Genetic Systems
Steven A. Benner, Nilesh B. Karalkar, Shuichi Hoshika, Roberto Laos, Ryan W. Shaw, Mariko Matsuura, Diego Fajardo, and Patricia Moussatche
Cold Spring Harb Perspect Biol , Cold Spring Harbor Laboratory Press (2016) doi: 10.1101/cshperspect.a023770


Polymerase Interactions with Wobble Mismatches in Synthetic Genetic Systems and Their Evolutionary Implications
Christian B. Winiger, Myong-Jung Kim, Shuichi Hoshika, Ryan W. Shaw, Jennifer D. Moses, Mariko F. Matsuura, Dietlind L. Gerloff, and Steven A. Benner
Biochemistry 55 (28) 3847-3850 (2016) DOI: 10.1021/acs.biochem.6b00533


A Single Deoxynucleoside Kinase Variant from Drosophila melanogaster Synthesizes Monophosphates of Nucleosides That Are Components of an Expanded Genetic System
Mariko F. Matsuura, Christian B. Winiger, Ryan W. Shaw, Myong-Jung Kim, Myong-Sang Kim, Ashley B. Daugherty, Fei Chen, Patricia Moussatche, Jennifer D. Moses, Stefan Lutz, and Steven A. Benner
ACS Synthetic Biology , American Chemical Society (2016) DOI: 10.1021/acssynbio.6b00228


Directed Evolution of Polymerases To Accept Nucleotides with Nonstandard Hydrogen Bond Patterns
Laos R, Shaw R, Leal NA, Gaucher E, Benner S.
Biochemistry (2013) 52, 5288-5294


Contribution of a conserved phenylalanine residue to the activity of Escherichia coli uracil DNA glycosylase
Shaw, RW; Feller, JA; Bloom, LB
DNA Repair 3 (10) 1273-1283 (2004)

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