Postdoctoral Fellow
Christian Winiger
Education
- BSc. Biology, Swiss Federal Institute of Technology (ETH), Zurich (2008)
- MSc. Chemical Biology, Swiss Federal Institute of Technology (ETH), Zurich (2010)
- PhD Chemistry and Molecular Science, University of Bern (2014)
- Postdoctoral Fellow, Foundation for Applied Molecular Evolution (2015)
Research summary
My research at FfAME focuses on the development of kinases for the phosphorylation of non-standard desoxyribonucleosides. The successful phosphorylation is tested in various enzyme-coupled assays and incorporation of non-standard nucleotide-triphosphates into DNA-templates by polymerases is probed. The long-term goal of the project is to create living systems which are able to process non-standard nucleosides and incorporate them into DNA and RNA.
Recent Publications
Polymerase Interactions with Wobble Mismatches in Synthetic
Genetic Systems and Their Evolutionary Implications
Christian B. Winiger, Myong-Jung Kim, Shuichi Hoshika, Ryan W. Shaw, Jennifer D. Moses, Mariko F. Matsuura, Dietlind L. Gerloff, and Steven A. Benner
Biochemistry
55 (28) 3847-3850 (2016) DOI: 10.1021/acs.biochem.6b00533
<Abstract>
In addition to completing the Watson-Crick nucleobase matching "concept" (big pairs with small,
hydrogen bond donors pair with hydrogen bond acceptors),
artificially expanded genetic information systems
(AEGIS) also challenge DNA polymerases with a
complete set of mismatches, including wobble mismatches.
Here, we explore wobble mismatches with AEGIS with
DNA polymerase 1 from Escherichia coli. Remarkably, we
find that the polymerase tolerates an AEGIS:standard
wobble that has the same geometry as the G:T wobble that
polymerases have evolved to exclude but excludes a wobble
geometry that polymerases have never encountered in
natural history. These results suggest certain limits to "structural analogy" and "evolutionary guidance" as tools
to help synthetic biologists expand DNA alphabets.
Formation of Two Homo-chromophoric H-Aggregates in DNA-Assembled Alternating Dye Stacks
C.B. Winiger; Dr. S.M. Langenegger; Prof. Dr. G. Calzaferri; Prof. Dr. R. Haner
Angew. Chem. Int. Ed.
(54) 3643-3647 (2015) DOI:10.1002/anie.201410041
Influence of perylenediimide-pyrene supramolecular interactions on the stability of DNA-based hybrids: Importance
of electrostatic complementarity
C.B. Winiger; Dr. S.M. Langenegger; Dr. O. Khorev; Prof. Dr. R. Haner
Beilstein J. Org. Chem.
(10) , Beilstein Institute 1589-1595 (2014) DOI:10.1002/anie.201407968
Long Distance EET in Light-Harvesting Supramolecular Polymers
C.B. Winiger; Dr. S. Li; Dr. G.R. Kumar; Dr. S.M. Langenegger; Prof. Dr. R. Haner
Angew. Chem. Int. Ed.
(53) 13609-13613 (2014) DOI:10.1002/anie.201407968
DOTA Conjugate with an Albumin-Binding Entity Enables the First Folic Acid-Targeted Lu-177-Radionuclide
Tumor Therapy in Mice
Dr. C. Mueller; Dr. H. Struthers; C. Winiger; Dr. K. Zhernosekov; Prof. Dr. R. Schibli
J. Nucl. Med.
(54) , Society of Nuclear Medicine and Molecular Imaging 124-131 (2013) DOI: 10.2967/jnumed.112.107235
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